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plasmid pet  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pet
    Plasmid Pet, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+expression+plasmids/pET-21a_3xNLS_SpCas9_protein_expression+(Plasmid+%23114365)/us12584145-563-5-10
    Average 94 stars, based on 21 article reviews
    plasmid pet - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs
    Article Snippet: The product plasmids were then transformed into Rosetta(DE3)pLysS Competent Cells (Millipore Sigma, Catalog #70956) following the manufacturer’s protocols. .. For LbCas12a, AsCas12a, FnCas12a, and MbCas12a, protein expression plasmids were obtained from Addgene and used directly for protein expression., , ..

    Article Title: Multi-pass, single-molecule nanopore reading of long protein strands.
    Article Snippet: .. Protein expression plasmids are available at Addgene. ..

    Article Title: Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs.
    Article Snippet: The product plasmids were then transformed into Rosetta(DE3)pLysS Competent Cells (Millipore Sigma, Catalog #70956) following the manufacturer’s protocols. .. For LbCas12a, AsCas12a, FnCas12a, and MbCas12a, protein expression plasmids were obtained from Addgene and used directly for protein expression.3,42,43 ..

    Article Title: Ligand-Enabled Selective Coupling of MIDA Boronates to Dehydroalanine-Containing Peptides and Proteins.
    Article Snippet: .. Protein expression plasmids (pET22b_PstS_1 and pNK0410 for the expression of PstS A197C and Ub S20C, respectively) were generous gifts from Dr. Martin Webb (shared via Addgene; plasmid #78198) and Dr. Naoya Kawakami. ..

    Article Title: The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition
    Article Snippet: The secondary antibodies were IRDye 800CW Goat Anti-Rabbit IgG antibody (Li-Cor #926–32211) for pS9-GSK3β and IRDye 680RD Donkey Anti-Mouse IgG antibody (Li-Cor #926–68072) for total GSK3β. pS9-GSK3β and total GSK3β levels were analyzed using Image Studio Lite 5.2.5 (Li-Cor). .. Protein expression plasmids ( ) generated in this work are available from Addgene or upon request. ..

    Article Title: Development and Recent Advances in SLIPT‐PM: A Chemogenetic Platform for Manipulating Signaling at the Plasma Membrane
    Article Snippet: .. [ ] Given its simplicity, the commercial availability of m D cTMP, and the deposition of iK6 DHFR‐tagged protein expression plasmids in Addgene, we anticipate that SLIPT‐PM will be widely adopted by researchers to manipulate cell signaling for various biological, biotechnological, and medical applications. ..

    Article Title: The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition
    Article Snippet: The secondary antibodies were IRDye 800CW Goat Anti- Rabbit IgG antibody (Li- Cor #926–32211) for pS9- GSK3β and IRDye 680RD Donkey Anti- Mouse IgG antibody (Li- Cor #926–68072) for total GSK3β. pS9- GSK3β and total GSK3β levels were analyzed using Image Studio Lite 5.2.5 (Li- Cor). .. Protein expression plasmids (Supplementary file 1f) generated in this work are available from Addgene or upon request. ..

    Article Title: 14-3-3 binding regulates Tau assembly and microtubule association
    Article Snippet: .. Protein expression plasmids are available upon request or available from Addgene. ..

    Plasmid Preparation:

    Article Title: Ligand-Enabled Selective Coupling of MIDA Boronates to Dehydroalanine-Containing Peptides and Proteins.
    Article Snippet: .. Protein expression plasmids (pET22b_PstS_1 and pNK0410 for the expression of PstS A197C and Ub S20C, respectively) were generous gifts from Dr. Martin Webb (shared via Addgene; plasmid #78198) and Dr. Naoya Kawakami. ..

    Generated:

    Article Title: The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition
    Article Snippet: The secondary antibodies were IRDye 800CW Goat Anti-Rabbit IgG antibody (Li-Cor #926–32211) for pS9-GSK3β and IRDye 680RD Donkey Anti-Mouse IgG antibody (Li-Cor #926–68072) for total GSK3β. pS9-GSK3β and total GSK3β levels were analyzed using Image Studio Lite 5.2.5 (Li-Cor). .. Protein expression plasmids ( ) generated in this work are available from Addgene or upon request. ..

    Article Title: The Axin scaffold protects the kinase GSK3β from cross-pathway inhibition
    Article Snippet: The secondary antibodies were IRDye 800CW Goat Anti- Rabbit IgG antibody (Li- Cor #926–32211) for pS9- GSK3β and IRDye 680RD Donkey Anti- Mouse IgG antibody (Li- Cor #926–68072) for total GSK3β. pS9- GSK3β and total GSK3β levels were analyzed using Image Studio Lite 5.2.5 (Li- Cor). .. Protein expression plasmids (Supplementary file 1f) generated in this work are available from Addgene or upon request. ..



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